af1389 (R&D Systems)
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Af1389, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dll4/Mouse+DLL4+Antibody/pmc13010106-234-2-3
Average 95 stars, based on 114 article reviews
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Real-time Polymerase Chain Reaction:Article Title: Bmp9 regulates Notch signaling and the temporal dynamics of angiogenesis via Lunatic Fringe Article Snippet: Antibodies against LFng (CST# 66472), Cleaved Notch1 (NICD CST# 4147), Jag1 (AF599) and β-actin were purchased from Cell Signaling, R&D systems and Santa Cruz Technologies respectively. .. Quantitect qPCR primers for Jag1 (QT00031948), Hey1 (QT00035644), Hey2 (QT00026971), Recombinant:Article Title: Bmp9 regulates Notch signaling and the temporal dynamics of angiogenesis via Lunatic Fringe Article Snippet: Antibodies against LFng (CST# 66472), Cleaved Notch1 (NICD CST# 4147), Jag1 (AF599) and β-actin were purchased from Cell Signaling, R&D systems and Santa Cruz Technologies respectively. .. Quantitect qPCR primers for Jag1 (QT00031948), Hey1 (QT00035644), Hey2 (QT00026971), Migration:Article Title: ERG activity is regulated by endothelial FAK coupling with TRIM25/USP9x in vascular patterning Article Snippet: .. After migration, proteins were transferred to a nitrocellulose membrane (Protran BA85, 0.45 μm; GE Healthcare Life Sciences) that was blocked with 5% nonfat milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies diluted in 4% BSA in TBS-T: FAK (Clone 77, 1:1000, 610287, BD Pharmingen), Membrane:Article Title: ERG activity is regulated by endothelial FAK coupling with TRIM25/USP9x in vascular patterning Article Snippet: .. After migration, proteins were transferred to a nitrocellulose membrane (Protran BA85, 0.45 μm; GE Healthcare Life Sciences) that was blocked with 5% nonfat milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies diluted in 4% BSA in TBS-T: FAK (Clone 77, 1:1000, 610287, BD Pharmingen), Saline:Article Title: ERG activity is regulated by endothelial FAK coupling with TRIM25/USP9x in vascular patterning Article Snippet: .. After migration, proteins were transferred to a nitrocellulose membrane (Protran BA85, 0.45 μm; GE Healthcare Life Sciences) that was blocked with 5% nonfat milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies diluted in 4% BSA in TBS-T: FAK (Clone 77, 1:1000, 610287, BD Pharmingen), Incubation:Article Title: ERG activity is regulated by endothelial FAK coupling with TRIM25/USP9x in vascular patterning Article Snippet: .. After migration, proteins were transferred to a nitrocellulose membrane (Protran BA85, 0.45 μm; GE Healthcare Life Sciences) that was blocked with 5% nonfat milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies diluted in 4% BSA in TBS-T: FAK (Clone 77, 1:1000, 610287, BD Pharmingen), Immunofluorescence:Article Title: Delta like 4 regulates cerebrovascular development and endothelial integrity via DLL4‐NOTCH‐CLDN5 pathway and is vulnerable to neonatal hyperoxia Article Snippet: Mouse anti-PECAM (CD31, #sc-376764; SCBT, Santa Cruz, CA, USA) was used in human brain staining. .. Immunofluorescence was used to determine the abundances of Marker:Article Title: Delta like 4 regulates cerebrovascular development and endothelial integrity via DLL4‐NOTCH‐CLDN5 pathway and is vulnerable to neonatal hyperoxia Article Snippet: Mouse anti-PECAM (CD31, #sc-376764; SCBT, Santa Cruz, CA, USA) was used in human brain staining. .. Immunofluorescence was used to determine the abundances of Enzyme-linked Immunosorbent Assay:Article Title: Allogeneic CAR T Cells Targeting DLL3 Are Efficacious and Safe in Preclinical Models of Small Cell Lung Cancer Article Snippet: Anti-DLL3 antibodies were first screened using a Flag-DLL3 (Adipogen) ELISA and then confirmed to target cellular DLL3 by flow cytometry against HEK-293T cells with or without human DLL3 expression. .. The antibodies were further counterscreened against structurally similar Notch ligands DLL1, Immunohistochemistry:Article Title: Kinetics of de novo Bone and Bone Marrow Niche Formation with Hybrid Click Cryogels Article Snippet: .. The following antibodies and reagents were used for immunohistochemistry: CD45 (ab10558, Abcam), NICD (ab52301, Abcam), |
