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af1389  (R&D Systems)


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    Structured Review

    R&D Systems af1389
    Af1389, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dll4/Mouse+DLL4+Antibody/pmc13010106-234-2-3
    Average 95 stars, based on 114 article reviews
    af1389 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Bmp9 regulates Notch signaling and the temporal dynamics of angiogenesis via Lunatic Fringe
    Article Snippet: Antibodies against LFng (CST# 66472), Cleaved Notch1 (NICD CST# 4147), Jag1 (AF599) and β-actin were purchased from Cell Signaling, R&D systems and Santa Cruz Technologies respectively. .. Quantitect qPCR primers for Jag1 (QT00031948), Hey1 (QT00035644), Hey2 (QT00026971), Dll4 (QT00081004), Hes1 (QT00039648), LFng (QT00212240), MFng (QT00084007), and RFng (QT01160075) were purchased from Qiagen, Recombinant BMP9 (3209-BP-010) and Dll4 (1506-D4-050) were obtained from R&D systems. .. HUVECs from Promocell (pooled donors, Cat#C-12203) were cultured with ECGM-2 (Lonza).

    Recombinant:

    Article Title: Bmp9 regulates Notch signaling and the temporal dynamics of angiogenesis via Lunatic Fringe
    Article Snippet: Antibodies against LFng (CST# 66472), Cleaved Notch1 (NICD CST# 4147), Jag1 (AF599) and β-actin were purchased from Cell Signaling, R&D systems and Santa Cruz Technologies respectively. .. Quantitect qPCR primers for Jag1 (QT00031948), Hey1 (QT00035644), Hey2 (QT00026971), Dll4 (QT00081004), Hes1 (QT00039648), LFng (QT00212240), MFng (QT00084007), and RFng (QT01160075) were purchased from Qiagen, Recombinant BMP9 (3209-BP-010) and Dll4 (1506-D4-050) were obtained from R&D systems. .. HUVECs from Promocell (pooled donors, Cat#C-12203) were cultured with ECGM-2 (Lonza).

    Migration:

    Article Title: ERG activity is regulated by endothelial FAK coupling with TRIM25/USP9x in vascular patterning
    Article Snippet: .. After migration, proteins were transferred to a nitrocellulose membrane (Protran BA85, 0.45 μm; GE Healthcare Life Sciences) that was blocked with 5% nonfat milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies diluted in 4% BSA in TBS-T: FAK (Clone 77, 1:1000, 610287, BD Pharmingen), DLL4 (1:1000, #] AF1389, R&D), Myc-tag clone 9E10 (1:1000, ab206486, Abcam), pY397-FAK (1:1000, 3283, CST), pY861-FAK (1:1000, PS1008, Invitrogen) and HSC70 (1: 5000, sc-7298, Santa Cruz Biotechnology). .. For ERG (1:500, ab9251, Abcam) and GAPDH (1:5000, MAB374, Millipore) immunoblotting, membranes were blocked with 3% nonfat milk in TBS for 1 h at room temperature and incubated overnight at 4°C with primary antibodies diluted in TBS-T. HRP-conjugated antibodies (1:1000, 111-035-003, 515-035-003, Jackson ImmunoResearch) were used for chemiluminescence detection with ECL western blotting (GE Healthcare) and protein levels were quantified by densitometry using ImageJ and normalised against loading controls HSC70 or GAPDH.

    Membrane:

    Article Title: ERG activity is regulated by endothelial FAK coupling with TRIM25/USP9x in vascular patterning
    Article Snippet: .. After migration, proteins were transferred to a nitrocellulose membrane (Protran BA85, 0.45 μm; GE Healthcare Life Sciences) that was blocked with 5% nonfat milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies diluted in 4% BSA in TBS-T: FAK (Clone 77, 1:1000, 610287, BD Pharmingen), DLL4 (1:1000, #] AF1389, R&D), Myc-tag clone 9E10 (1:1000, ab206486, Abcam), pY397-FAK (1:1000, 3283, CST), pY861-FAK (1:1000, PS1008, Invitrogen) and HSC70 (1: 5000, sc-7298, Santa Cruz Biotechnology). .. For ERG (1:500, ab9251, Abcam) and GAPDH (1:5000, MAB374, Millipore) immunoblotting, membranes were blocked with 3% nonfat milk in TBS for 1 h at room temperature and incubated overnight at 4°C with primary antibodies diluted in TBS-T. HRP-conjugated antibodies (1:1000, 111-035-003, 515-035-003, Jackson ImmunoResearch) were used for chemiluminescence detection with ECL western blotting (GE Healthcare) and protein levels were quantified by densitometry using ImageJ and normalised against loading controls HSC70 or GAPDH.

    Saline:

    Article Title: ERG activity is regulated by endothelial FAK coupling with TRIM25/USP9x in vascular patterning
    Article Snippet: .. After migration, proteins were transferred to a nitrocellulose membrane (Protran BA85, 0.45 μm; GE Healthcare Life Sciences) that was blocked with 5% nonfat milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies diluted in 4% BSA in TBS-T: FAK (Clone 77, 1:1000, 610287, BD Pharmingen), DLL4 (1:1000, #] AF1389, R&D), Myc-tag clone 9E10 (1:1000, ab206486, Abcam), pY397-FAK (1:1000, 3283, CST), pY861-FAK (1:1000, PS1008, Invitrogen) and HSC70 (1: 5000, sc-7298, Santa Cruz Biotechnology). .. For ERG (1:500, ab9251, Abcam) and GAPDH (1:5000, MAB374, Millipore) immunoblotting, membranes were blocked with 3% nonfat milk in TBS for 1 h at room temperature and incubated overnight at 4°C with primary antibodies diluted in TBS-T. HRP-conjugated antibodies (1:1000, 111-035-003, 515-035-003, Jackson ImmunoResearch) were used for chemiluminescence detection with ECL western blotting (GE Healthcare) and protein levels were quantified by densitometry using ImageJ and normalised against loading controls HSC70 or GAPDH.

    Incubation:

    Article Title: ERG activity is regulated by endothelial FAK coupling with TRIM25/USP9x in vascular patterning
    Article Snippet: .. After migration, proteins were transferred to a nitrocellulose membrane (Protran BA85, 0.45 μm; GE Healthcare Life Sciences) that was blocked with 5% nonfat milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies diluted in 4% BSA in TBS-T: FAK (Clone 77, 1:1000, 610287, BD Pharmingen), DLL4 (1:1000, #] AF1389, R&D), Myc-tag clone 9E10 (1:1000, ab206486, Abcam), pY397-FAK (1:1000, 3283, CST), pY861-FAK (1:1000, PS1008, Invitrogen) and HSC70 (1: 5000, sc-7298, Santa Cruz Biotechnology). .. For ERG (1:500, ab9251, Abcam) and GAPDH (1:5000, MAB374, Millipore) immunoblotting, membranes were blocked with 3% nonfat milk in TBS for 1 h at room temperature and incubated overnight at 4°C with primary antibodies diluted in TBS-T. HRP-conjugated antibodies (1:1000, 111-035-003, 515-035-003, Jackson ImmunoResearch) were used for chemiluminescence detection with ECL western blotting (GE Healthcare) and protein levels were quantified by densitometry using ImageJ and normalised against loading controls HSC70 or GAPDH.

    Immunofluorescence:

    Article Title: Delta like 4 regulates cerebrovascular development and endothelial integrity via DLL4‐NOTCH‐CLDN5 pathway and is vulnerable to neonatal hyperoxia
    Article Snippet: Mouse anti-PECAM (CD31, #sc-376764; SCBT, Santa Cruz, CA, USA) was used in human brain staining. .. Immunofluorescence was used to determine the abundances of DLL4 (#MAB1389-SP; R&D Systems) and CLDN5 (#PA5-99415; Thermo Fisher) as well the colocalizations of DLL4 with either CD31 or GFAP (astrocyte marker, #PA110004; Thermo Fisher) or NeuN (neuronal marker, #24307; Cell Signaling) or CD11b (microglia marker, #17800S; Cell Signaling) or nestin (stem cell marker, #14-5843-82; Thermo Fisher) or Sox 10 (oligodendrocyte marker, #NBP2-76378; Novus) in P7 CD1 WT brain. .. Images were captured using a BZ-X800 microscope (Keyence Itasca, IL, USA) at 20× magnification.

    Marker:

    Article Title: Delta like 4 regulates cerebrovascular development and endothelial integrity via DLL4‐NOTCH‐CLDN5 pathway and is vulnerable to neonatal hyperoxia
    Article Snippet: Mouse anti-PECAM (CD31, #sc-376764; SCBT, Santa Cruz, CA, USA) was used in human brain staining. .. Immunofluorescence was used to determine the abundances of DLL4 (#MAB1389-SP; R&D Systems) and CLDN5 (#PA5-99415; Thermo Fisher) as well the colocalizations of DLL4 with either CD31 or GFAP (astrocyte marker, #PA110004; Thermo Fisher) or NeuN (neuronal marker, #24307; Cell Signaling) or CD11b (microglia marker, #17800S; Cell Signaling) or nestin (stem cell marker, #14-5843-82; Thermo Fisher) or Sox 10 (oligodendrocyte marker, #NBP2-76378; Novus) in P7 CD1 WT brain. .. Images were captured using a BZ-X800 microscope (Keyence Itasca, IL, USA) at 20× magnification.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Allogeneic CAR T Cells Targeting DLL3 Are Efficacious and Safe in Preclinical Models of Small Cell Lung Cancer
    Article Snippet: Anti-DLL3 antibodies were first screened using a Flag-DLL3 (Adipogen) ELISA and then confirmed to target cellular DLL3 by flow cytometry against HEK-293T cells with or without human DLL3 expression. .. The antibodies were further counterscreened against structurally similar Notch ligands DLL1, DLL4, Jagged-1, and Jagged-2 (R&D systems) using ELISA. .. HEK293T cells were transfected with the CAR construct along with psPAX2 and pMD.2G plasmids at a ratio of 1:3:1, respectively, using Lipofectamine2000 (Invitrogen) according to the manufacturer’s protocol to produce lentivirus supernatant.

    Immunohistochemistry:

    Article Title: Kinetics of de novo Bone and Bone Marrow Niche Formation with Hybrid Click Cryogels
    Article Snippet: .. The following antibodies and reagents were used for immunohistochemistry: CD45 (ab10558, Abcam), NICD (ab52301, Abcam), DLL4 (AF1389, R&D systems), Hoechst 33258 (H21491, Invitrogen), and Prolong Gold antifade reagent (Invitrogen). .. The sections were imaged with a ZEISS Axio Scan.Z1 Slide Scanner in bright-field mode.



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    Image Search Results


    (a) αSMA mRNA levels in HCAEC treated with TGFβ1 (10 ng/ml) or exposed to hypoxic conditions (5% O 2 ), in the absence or presence of moderate dose EtOH (25 mM, green bars) or high dose EtOH (100 mM, red bars) +/- the γ-secretase inhibitor DAPT (20 μM). (b) αSMA and fibronectin (FN) mRNA levels in HCAEC treated with TGFβ1 +/- the Notch ligand DLL4 (3 μg/ml) in the absence or presence of DAPT (20 μM). Data are mean±SEM, n=3. *p<0.05 vs control (no treatment), #p<0.05 vs TGFβ1 or hypoxia.

    Journal: bioRxiv

    Article Title: A Biphasic Effect of Alcohol on Endothelial Plasticity Through Regulation of Endothelial-to-Mesenchymal Transition

    doi: 10.64898/2026.04.14.718463

    Figure Lengend Snippet: (a) αSMA mRNA levels in HCAEC treated with TGFβ1 (10 ng/ml) or exposed to hypoxic conditions (5% O 2 ), in the absence or presence of moderate dose EtOH (25 mM, green bars) or high dose EtOH (100 mM, red bars) +/- the γ-secretase inhibitor DAPT (20 μM). (b) αSMA and fibronectin (FN) mRNA levels in HCAEC treated with TGFβ1 +/- the Notch ligand DLL4 (3 μg/ml) in the absence or presence of DAPT (20 μM). Data are mean±SEM, n=3. *p<0.05 vs control (no treatment), #p<0.05 vs TGFβ1 or hypoxia.

    Article Snippet: Where indicated, cells were treated with Notch inhibitor DAPT (20 μM, Cat. #2634, Tocris,) or Notch ligand DLL4 (2 μg/ml, Cat. # 1506-D4, R&D Systems).

    Techniques: Control